Particle size measurement of lipoprotein fractions using diffusion-ordered NMR spectroscopy

Abstract The sizes of certain types of lipoprotein particles have been associated with an increased risk of cardiovascular disease. However, there is currently no gold standard technique for the determination of this parameter. Here, we propose an analytical procedure to measure lipoprotein particles sizes using diffusion-ordered nuclear magnetic resonance spectroscopy (DOSY). The method was tested […]

Metabolomics Reveals Reduction of Metabolic Oxidation in Women with Polycystic Ovary Syndrome after Pioglitazone-Flutamide-Metformin Polytherapy

Abstract Polycystic ovary syndrome (PCOS) is a variable disorder characterized by a broad spectrum of anomalies, including hyperandrogenemia, insulin resistance, dyslipidemia, body adiposity, low-grade inflammation and increased cardiovascular disease risks. Recently, a new polytherapy consisting of low-dose flutamide, metformin and pioglitazone in combination with an estro-progestagen resulted in the regulation of endocrine clinical markers in […]

Surface fitting of 2D diffusion-edited 1H NMR spectroscopy data for the characterisation of human plasma lipoproteins

Abstract Determining the concentration and size of lipoprotein complexes is very important due to their role in cardiovascular diseases and metabolic disorders. However, standard methods for lipoprotein fractionation are manual and time consuming and cannot be used as standard diagnostic tools. Because different subclasses of lipoproteins have different radii and, hence, different diffusion velocities, we […]