Current status and challenges of multi-omics research using animal models of atherosclerosis
Abstract Atherosclerosis is an underlying cause of cardiovascular diseases (CVD) which account for most deaths worldwide. Use of diverse preclinical models of atherosclerosis has been implemental in understanding the underlying mechanisms, the implicated cell types, the genes and the molecules at play in the onset and progression of atherosclerotic plaques. Although significant research advancements have […]
Microbiota Shape Metabolic and Immune Determinants of CAR-T Therapy and Correlate with Outcomes in Myeloma
Abstract Multiple myeloma remains incurable despite advances in immunotherapies like chimeric antigen receptor (CAR) T-cell therapy. This study investigates the role of metabolites and gut microbiota in clinical outcomes in patients treated with the humanized B-cell maturation antigen (BCMA)-directed CAR-T therapy ARI0002h. Stool metabolites, particularly succinate, were associated with CAR T-cell phenotypes and persistence in […]
ApoC-III proteoforms are associated with better lipid, inflammatory, and glucose profiles independent of total apoC-III
Abstract Background: Apolipoprotein (apo) C-III is involved in several processes that increase triglyceride levels, inflammation, and insulin resistance. Four of its proteoforms have been the focus of several studies and have shown differential associations with cardiovascular risk biomarkers, mostly lipids. However, there are other proteoforms of apoC-III that have not yet been investigated in detail. […]
Glycoprotein Determination with NMR Spectroscopy to Evaluate the Effect of Metabolic Surgery over the Proinflammatory State of Patients with Obesity
Abstract Aim: Patients with obesity present with a proinflammatory state generally quantified by measuring acute-phase proteins (APP) such as C reactive protein (CPR) which shows however great variability. The determination of glycoproteins by using MRI spectroscopy, specifically acetyl groups of N-acetylglucosamine and N-acetylgalactosamine (GlycA), and acetyl groups of N-acetylneuraminic acid (GlycB), allows us to measure […]
Triglycerides as Determinants of Global Lipoprotein Derangement: Implications for Cardiovascular Prevention
Abstract Plasma triglyceride levels are a strong cardiovascular risk marker. However, triglyceride-lowering therapies have not demonstrated a reduction in cardiovascular events, suggesting that triglycerides may serve as surrogate markers for other atherogenic mechanisms. The aim is to investigate the role of triglycerides in derangements of the global lipoprotein profile, as assessed by 1H-NMR spectroscopy. Serum […]
Functional and metabolomic characterization of human cell models to address obesity and metabolic dysfunction- associated steatotic liver disease pathogenetic mechanisms
Abstract Introduction and aim. Obesity and metabolic dysfunction-associated steatotic liver disease (MASLD) are global health concerns linked to an increased risk of atherosclerotic cardiovascular disease (ASCVD). Despite progresses in elucidating relevant pathophysiological mechanisms involved in obesity and MASLD, a comprehensive understanding toward ASCVD risk is missing due to the lack of reliable in vitro models […]
The multiomics blueprint of the individual with the most extreme lifespan
Abstract Extreme human lifespan, exemplified by supercentenarians, presents a paradox in understanding aging: despite advanced age, they maintain relatively good health. To investigate this duality, we have performed a high-throughput multiomics study of the world’s oldest living person, interrogating her genome, transcriptome, metabolome, proteome, microbiome, and epigenome, comparing the results with larger matched cohorts. The […]
Long-term mitochondrial and metabolic impairment in lymphocytes of subjects who recovered after severe COVID-19
Abstract The underlying mechanisms explaining the differential course of SARS-CoV-2 infection and the potential clinical consequences after COVID-19 resolution have not been fully elucidated. As a dysregulated mitochondrial activity could impair the immune response, we explored long-lasting changes in mitochondrial functionality, circulating cytokine levels, and metabolomic profiles of infected individuals after symptoms resolution, to evaluate […]
Advanced serum lipoprotein and glycoprotein profiling for cardiovascular event prediction in type 2 diabetes mellitus: the LIPOCAT study
Abstract Background: Traditional risk factors cannot accurately predict cardiovascular events (CVE) in type 2 diabetes (T2D). The LIPOCAT study aimed to prospectively evaluate the clinical utility of advanced lipoprotein characteristics and glycoproteins to predict future cardiovascular events (CVE) in a large cohort of subjects with type 2 diabetes mellitus (T2D). Methods: From four different Spanish […]
Ketone Bodies Are Potential Prognostic Biomarkers in Relapsed/Refractory Diffuse Large B-Cell Lymphoma: Results from the R2-GDP-GOTEL Trial
Abstract Background: Patients with relapsed or refractory (R/R) diffuse large B-cell lymphoma (DLBCL) who are ineligible for high-dose chemotherapy have limited treatment options and poor life expectancy. The purpose of this study is to identify a serum metabolomic profile that may be predictive of outcome in patients with R/R-DLBCL. Methods: This study included 69 R/R […]
