The multiomics blueprint of the individual with the most extreme lifespan
Abstract Extreme human lifespan, exemplified by supercentenarians, presents a paradox in understanding aging: despite advanced age, they maintain relatively good health. To investigate this duality, we have performed a high-throughput multiomics study of the world’s oldest living person, interrogating her genome, transcriptome, metabolome, proteome, microbiome, and epigenome, comparing the results with larger matched cohorts. The […]
Glycoprotein Determination with NMR Spectroscopy to Evaluate the Effect of Metabolic Surgery over the Proinflammatory State of Patients with Obesity
Abstract Aim: Patients with obesity present with a proinflammatory state generally quantified by measuring acute-phase proteins (APP) such as C reactive protein (CPR) which shows however great variability. The determination of glycoproteins by using MRI spectroscopy, specifically acetyl groups of N-acetylglucosamine and N-acetylgalactosamine (GlycA), and acetyl groups of N-acetylneuraminic acid (GlycB), allows us to measure […]
Triglycerides as Determinants of Global Lipoprotein Derangement: Implications for Cardiovascular Prevention
Abstract Plasma triglyceride levels are a strong cardiovascular risk marker. However, triglyceride-lowering therapies have not demonstrated a reduction in cardiovascular events, suggesting that triglycerides may serve as surrogate markers for other atherogenic mechanisms. The aim is to investigate the role of triglycerides in derangements of the global lipoprotein profile, as assessed by 1H-NMR spectroscopy. Serum […]
Functional and metabolomic characterization of human cell models to address obesity and metabolic dysfunction- associated steatotic liver disease pathogenetic mechanisms
Abstract Introduction and aim. Obesity and metabolic dysfunction-associated steatotic liver disease (MASLD) are global health concerns linked to an increased risk of atherosclerotic cardiovascular disease (ASCVD). Despite progresses in elucidating relevant pathophysiological mechanisms involved in obesity and MASLD, a comprehensive understanding toward ASCVD risk is missing due to the lack of reliable in vitro models […]
Current status and challenges of multi-omics research using animal models of atherosclerosis
Abstract Atherosclerosis is an underlying cause of cardiovascular diseases (CVD) which account for most deaths worldwide. Use of diverse preclinical models of atherosclerosis has been implemental in understanding the underlying mechanisms, the implicated cell types, the genes and the molecules at play in the onset and progression of atherosclerotic plaques. Although significant research advancements have […]
Microbiota Shape Metabolic and Immune Determinants of CAR-T Therapy and Correlate with Outcomes in Myeloma
Abstract Multiple myeloma remains incurable despite advances in immunotherapies like chimeric antigen receptor (CAR) T-cell therapy. This study investigates the role of metabolites and gut microbiota in clinical outcomes in patients treated with the humanized B-cell maturation antigen (BCMA)-directed CAR-T therapy ARI0002h. Stool metabolites, particularly succinate, were associated with CAR T-cell phenotypes and persistence in […]
Multiomics in atherosclerotic cardiovascular disease
Abstract Background and aims: Atherosclerotic cardiovascular disease (ASCVD) is among the leading causes of death worldwide, and technological advances have made it possible to expand the repertoire of biomarkers used in diagnostics and treatment of ASCVD. These include different omics (genomics, epigenomics, transcriptomics, proteomics, and metabolomics). We introduce the various layers of omic data and […]
Initiating Empagliflozin and Sacubitril/Valsartan Early After Acute Myocardial Infarction: Mechanistic Study
Abstract Background: Empagliflozin and sacubitril/valsartan are established in heart failure treatment, but their effects after myocardial infarction (MI) are less clear. This study evaluated early empagliflozin initiation, with or without sacubitril/valsartan, on post-MI inflammation, oxidative stress, metabolism, fibrosis, cardiac function, and ventricular tachycardia (VT) risk in a pig model. Methods: A total of 24 of […]
Urinary Hippuric Acid as a Sex-Dependent Biomarker for Fruit and Nut Intake Raised from the EAT-Lancet Index and Nuclear Magnetic Resonance Analysis
Abstract Background/Objectives: Assessing nutrient intake is essential for understanding body homeostasis and diet-health interactions. Traditional methods, such as dietary questionnaires and quality indices, are limited by subjectivity and variability in food composition tables. Metabolomic markers, like urinary hippuric acid, provide an objective means to estimate food and nutrient intake, helping to link dietary patterns with […]
Fundamentals of lipoprotein(a) request and quantification in the clinical laboratory
Abstract Cardiovascular diseases keep being the leading cause of mortality in Spain. Efforts should be intensified to identify new risk factors that may contribute to increasing cardiovascular risk. Lipoprotein(a) (Lp(a)) has been associated with a higher risk for developing aortic valve stenosis, heart failure, ischemic stroke, ischemic heart disease and peripheral arterial disease. Hyperlipoproteinemia(a) is […]